The sterile discs were dipped in silver nanoparticles solution 10mgml and placed in the. Antimicrobial activity of metal oxide nanoparticles against grampositive and gramnegative bacteria. Antibacterial activity of zinc oxide and ag doped zinc oxide. The importance of their application as antibacterial agents is evident keeping in mind the limited range and effectiveness of antibiotics, on one hand, and the plethora of metal oxides, on the other. Synthesis,characterization and antibacterial properties of zinc oxide nanoparticles 47 particle size, morphology and structure 3537. Antibacterial activity and mechanism of zno nanoparticles on.
Synthesis and characterization of zno nanoparticles for biomedical applications zargar ra1 and arora m2 1department of physics, g. Synthesis and characterization of zinc and copper oxide. Taking advantage of zinc oxide zno and graphene oxide go, the znogo composites were prepared by a facile onepot reaction to achieve superior antibacterial properties without damaging other species. Different parameters effect on the nps antibacterial activity antibacterial activity and wastewater disinfection using cuo nps was studied using different parameters. Cuo and fe2o3 as antibacterial agents against multidrug resistant biofilm forming bacteria was evaluated. Effects of longterm exposure to zinc oxide nanoparticles. Antibacterial activity of zno nanoparticle on grampositive and gramnegative bacteria article pdf available in african journal of microbiology research 518 june 2012 with 10,031 reads. Antibacterial activity and mechanism of action of zinc oxide. Zinc oxide nanoparticles znonps are among inorganic metal oxide and are considered a safe material being significantly used for antibacterial activity due to their physical and chemical properties 4. Padmavathy3 1school of bioscience and biotechnology, 2jadavpur university, kolkata, 3centre for nanomaterials, school of advanced sciences, vit university, vellore 632014, tamilnadu, india 7klv. New materials with good antibacterial activity and less toxicity to other species attract numerous research interest. Synthesis and characterization of zno nanoparticles.
Zinc oxide nanoparticles were synthesized using a simple precipitation method with zinc sulfate and sodium hydroxide as starting materials. Synthesis, characterization and enhanced antimicrobial. Another application of copper oxide cuo nps for antimicrobial applications was. Concerning the biosafety of nanotechnology, nanotoxicity is going to be the second most priority of nanotechnology that. Antibacterial activity the antibacterial activities of snps were carried out by disc diffusion method14. During drying, complete conversion of zinc hydroxide into zinc oxide takes place. Hazardous effects of titanium dioxide nanoparticles in ecosystem. Overall, the antimicrobial effect of trimetal nps is between those of pure zno and cuo nanoparticles, which may mean that their cytotoxicity is. The samples were characterized by xray diffraction xrd, scanning electron microscopy sem, energy dispersive spectroscopy eds, and protoninduced xray emission pixe analysis. In the composites, zno nanoparticles nps, with a size of about 4 nm. Therefore, we aimed in this study to test the antibacterial activity of zno nanoparticles on important and perilous bacteria such as p. Antibacterial activity of silver and zinc nanoparticles. Since zinc oxide nanoparticles are characterised by their uva and uvb protection properties as well as by the previously described transparency and antibacterial and fungistatic effects, they are also used in textiles, clear varnishes for wood products and furniture, and in transparent plastics and plastic films plastic glasses.
Zno nanoparticles can be obtained by physical and chemical routes. The sizes of the zones of growth inhibition are presented in table 1. Photocatalytically active zinc oxide and titanium dioxide. The effects of zinc oxide and silver nanoparticles on intestinal bacteria ami yoo dr.
Antibacterial activity of zinc oxide and ag doped zinc. Whiles zinc oxide nanoparticles were spherical of average diameter of 15 nm. Antibacterial activity and mechanism of action of zinc. Pdf antibacterial activity of zinc oxide nanoparticles.
The aqueous extract of lycopersicon esculentum red tomato was used for the rapid synthesis of agnp, which is very simple and. Zno nanoparticles against pathogenic bacteria and fungi. The importance of their application as antibacterial agents is evident keeping in mind the limited range and effectiveness of antibiotics, on one hand, and the plethora of metal oxides, on the other, along with the. Wastewater disinfection by synthesized copper oxide. Antibacterial activity of zinc oxidenanoparticles znonps has received significant interest worldwide particularly by the implementation of nanotechnology to synthesize particles in the nanometer region. Synthesis of zinc oxide nanoparticles and evaluated its. However, the antibacterial concentrations were inconsistent.
Pdf antibacterial activity of zno nanoparticle on gram. These nanoparticles have a great potential in the management of plant diseases as compared to synthetic fungicides park et al. Nanoparticle, xrd, uvvis absorption, antibacterial i. In this study, pure and single phase nio and ni nps were successfully synthesized by a simple and costeffective boiling method using leaves of azadirachta indica and psidium guajava. Oct 17, 2012 the development of reliable processes for the synthesis of zinc oxide nanoparticles is an important aspect of nanotechnology today.
Hazardous effects of titanium dioxide nanoparticles in. The tem micrographs showed that copper oxide nanoparticles assumed a nanorod shape of average length of 100 nm. Antibacterial activity of zinc oxide nanoparticles on staphylococcus aureus article pdf available april 2016 with 2,357 reads how we measure reads. Znonps exhibit attractive antibacterial properties due to increased specific surface area as the. Antimicrobial behavior of zinc oxide nanoparticles and. The antibacterial activity of zno is dependent on the size and concentration of the particles. Song and kim, 2009, resulting in the four different types of nanoparticles znonpsl, znonpsf, agnpsl. The xrd analysis revealed that both zinc and copper oxide nanoparticles were purely crystalline. Antibacterial activity preparation of nanosuspension synthesized nanoparticles was suspended in 1 ml of dilute. In particular, zinc oxide and titanium dioxide nanoparticles 3, 4 figs.
Antibacterial activity of trimetal cuznfe oxide nanoparticles ncbi. Zinc oxide nano particles are better antibacterial agent 2122. Antibacterial activity of silver nanoparticles synthesized. Zinc oxide zno nanoparticles have received a remarkable consideration due to their extra ordinary uv filtering properties, photochemical antifungal, high catalytic and antibacterial activities. The zno nanoparticles were prepared by wet chemical method and were directly applied on to the 100% cotton woven fabric using paddrycure method. A unique and profound effect of mgo and zno nanoparticles. Antibacterial activity of silver and zinc nanoparticles against vibrio cholerae and enterotoxic escherichia coli author links open overlay panel wesam salem a b deborah r. Zno nanoparticles have significantly higher antibacterial effect on s. In this study, we have investigated the antimicrobial effects of silver nanoparticles prepared by a biosynthesis method. It is one of the cheap materials in cosmetic industry.
The advent of nanotechnology has led the development of materials with new properties for use as antimicrobial agents. Zinc oxide and silver nanoparticles znonps and agnps were synthesized according to established protocols using leaf l and fruit extracts f from c. Zingl a 1 gebhart schratter c ruth prassl c walter goessler d joachim reidl a stefan schild a. Characterization and antibacterial activity of iron oxide nanoparticles 336 synthesis of iron oxide nanoparticles the magnetic nanoparticles synthesized by coprecipitation method specifically based on parameters such as reaction temperature, ph of the suspension, initial molar concentration etc. But the ag doped zinc oxide nanoparticles showed better antibacterial activity than the zno nanoparticles. Many microorganisms exist in the range from hundreds of nanometers to tens of micrometers. Zinc oxide nanoparticles nanoznos are widely used and possess great potentials in agriculture and biomedicine. Use of zinc oxide nano particles for production of. When the size is 12 nm, it inhibits the growth of s. Over the past decade, incorporation of nanomaterials into agricultural practices like nanofertilizers and nanopesticides has gained a lot of attention. Antibacterial activity of silver nanoparticles synthesized by sidr. Antimicrobial activity of zno nanoparticles against.
The antibacterial effect of zinc oxide zno nanoparticles on campylobacter jejuni was investigated for inhibition and inactivation of cell growth. The antibacterial activity of zno nanoparticles was evaluated by measuring the zone of inhibition against the test organisms. Synthesis of metal nanoparticles with antimicrobial properties has become a viable alternative and has. It has been reported that materials based on zno nanoparticles can be used in infrared and elect rochemical sensors 28, 29. One of the main health problems is increasing resistance of bacteria to antimicrobial agents worldwide. Emergence of antimicrobial resistance by pathogenic bacteria is a major health problem in recent years. Antibacterial activity and toxicity mechanism article pdf available april 2015 with 2,109 reads how we measure reads. Biosynthesis and antibacterial activity of zno nanoparticles. The antibacterial activity of the finished fabrics was assessed qualitatively by agar diffusion and parallel streak method, quantitatively by percentage reduction test. Tem and sem images clearly indicated the presence of graphene sheets decorated with uniformly sized zinc oxide nanoparticles. Synthesis, characterization and optical properties of zinc. Antibacterial activity of zinc oxide nanoparticles on staphylococcus aureus.
Nanoparticles and nanomaterials are currently considered to be new antibiotics. Abstract with an increase in antibiotic resistance, a growing interest in developing new antimicrobial agents has gained popularity. Biosynthesis of nio and ni nanoparticles and their. Boys khanabal, india 2csirnational physical laboratory, dr. Pdf antibacterial activity of zinc oxide nanoparticles on.
Zinc oxide nanoparticles zno nps are considered as a biosafe material for biological. This study aimed at investigating effects of nanoznos on development, zinc metabolism and biodistribution of. To study the role of temperature and sodium hydroxide. The development of reliable processes for the synthesis of zinc oxide nanoparticles is an important aspect of nanotechnology today. It is inevitable for human exposure to these nanoparticles. Further, as an application of rgozno nanocomposites, their superior antibacterial activity was demonstrated on the escherichia coli mtcc40 bacterial strain, using the standard disk diffusion method. Synthesis of metal oxide nanoparticles nps and its deposition onto cotton fibers were conducted using various methods. Humans quest for innovation, finding solutions of problems, and upgrading the industrial yield with energy efficient and costeffective materials has opened the avenues of nanotechnology.
Initial cell concentration was around 109 cfu ml1, and aqueous znonps suspensions ranged. The weak band near 1598 cm1 is assigned to hoh bending vibration mode were presented due to the adsorption of moisture, when ftir. The antibacterial activity was performed by kirbys disc diffusion assay using different concentrations 0. Introduction zinc oxide and cadmium sulphide nanoparticles have received much attention for their implications in. It has been known for quite some time now that silver nanoparticles agnp can inhibit microbial growth and even kill microbes. Zinc oxide zno and magnesium oxide mgo nanoparticles are an effective antibacterial and antiodor agent shah and towkeer, 2010. However, chemical methods are more suitable for industrial scale production 35 due to its low efficiency and. Nanoparticles nps, such as metals, carbon nanotubes cnts and polymers, are structures having a diameter of 1100 nm in at least one dimension. Nov, 2014 the antibacterial activity of agnp is significantly enhanced when it is modified with sodium dodecyl sulfate sds kevitec et al. The results show that the zno nanoparticles have bacteriostatic activity against e. Silver nanoparticles had superior antibacterial activity than. Global ournal of anomedicine in addition to these phonon peaks, two phonon peak of 2e. In vitro antibacterial activity of zno and nd doped zno nanoparticles against esbl producing escherichia coli and klebsiella pneumoniae. Antimicrobial activities of silver nanoparticles synthesized.
Anti bacterial activity of free zinc oxide reveals free nanoparticles inhibited all the tested bacteria. Feb 04, 2011 antibacterial tests both on agar plates and in broth showed that 0. Nanoparticles can be useful for preventing the distribution of resistant bacteria in the hospital and community. After addition of zno, zno topo, and znobrij76 nanoparticles h 10, a progressive decrease of photosynthetic activity was observed in the first 10 days for both microorganisms anabaena flosaquae, after 10 days of incubation, an increase of the photosynthetic activity was observed after contact with zno and znobrij76 nanoparticles. This article elaborates on the properties and applications of zinc oxide nanoparticles. Comparative study of antibiofilm activity of copper oxide and. Apr 19, 2015 antibacterial activity of zinc oxide nanoparticles znonps has received significant interest worldwide particularly by the implementation of nanotechnology to synthesize particles in the nanometer region.
The results indicated that zno nanoparticles synthesized from t. Zno is currently listed as a generally recognized as safe gras material by the food and drug administration and is used as food additive. Znonps exhibit attractive antibacterial properties due to increased. Zinc oxide nanoparticles zno nps are considered as a biosafe. Vibrio cholerae and enterotoxic escherichia coli etec remain two dominant bacterial causes of severe secretory diarrhea and still a significant cause of death, especially in developing countries. Our investigation reports the antimicrobial activity of agnp against a model bacterium, escherichia coli. The effectiveness of the metal oxide nanoparticles viz. Comparative study of antibiofilm activity of copper oxide. Among a variety of nanoparticles, zinc oxide nanoparticles zno have advantages because of the extraordinary physical and chemical properties. The samples were characterized by xray diffraction xrd, scanning electron microscopy sem, energy dispersive spectroscopy eds, and proton. Antibacterial activity of zinc oxide nanoparticles znonps has received significant interest worldwide particularly by the implementation of nanotechnology to synthesize particles in the nanometer region.
The antibacterial activity against li was investigated under photocatalytic light setup equipped with 250watts halogen lamp. Many researchers have reported that zno nps, as an antibacterial factor for pathogenic microbes 5, 6, are currently. Zinc is a block d, period 4 element while oxygen is a block p, period 2 element. Zinc oxide nanoparticles prepared from the solution of zinc acetate, peg and ammonium carbonate. These nanoparticles exhibit antibacterial, anticorrosive, antifungal and uv filtering properties. Mar 12, 2014 the effectiveness of the metal oxide nanoparticles viz. The parameters were nps size effect, the presence and absence of toab surfactant, nanoparticles concentrations, contact time, ph, shaking and temperature of wastewater effect. Synthesis of nanometal oxidecoated cotton composites. Zinc oxide zno is an inorganic compound widely used in everyday applications.
Washing, the nanoparticles were dried at 80 oc for overnight. In our earlier work we have studied the antibacterial property of zinc oxide nanoparticles synthesized using zinc acetate precursor and its application in bandages has been studied 11. The absorption band at 452 cm1 was assigned to zno stretching vibration 10. Antibacterial activity of zinc oxide nanoparticles synthesized by solochemical process brailian journal of chemical ngineering, ol. Zinc oxide zno is a provocative material for applications in electronics, photonics, acoustics and sensing. After solidification bacterial cultures were swabbed on these plates. Th antibacterial activity of metal oxide nanoparticles has received marked global attention as they can be specifically synthesized to exhibit significant toxicity to bacteria.
Here we get a new line of high purity zinc oxide nanoparticles primarily targeted for antimicrobial activity against bacillus subtilis and escherichia coli. Azlin mustapha, thesis supervisor abstract in this study, the effects of zinc oxide zno and silver ag nanoparticles nps on the intestinal bacteria, escherichia coli, lactobacillus acidophilus, and bifidobacterium animalis were investigated. Zinc oxide zno nanopowders are available as powders and dispersions. The synthesized sample was calcined at different temperatures for 2 h. Copper nanoparticles as antibacterial agents omics international. Thus, zno in nanoscale has shown antimicrobial properties. Zinc oxide nanoparticles comprise wellknown inhibitory and bactericidal effects. Progress and application of fertilizers in nanoforms are one of the effective options for considerable improvement of the agricultural yield worldwide. Antibacterial effects of zinc oxide nanoparticles on. Apr, 2016 in vitro antibacterial activity of zno and nd doped zno nanoparticles against esbl producing escherichia coli and klebsiella pneumoniae. Properties of zinc oxide nanoparticles and their activity.
Nutrient agar medium plates were prepared, sterilized and solidified. Antimicrobial activity of metal oxide nanoparticles against gram. The present study is concerned about the synthesis, characterization of zinc. Superior antibacterial activity of zinc oxidegraphene. Synthesis, characterization, and antimicrobial activity of. Synthesis and characterization of zno nanoparticles for biomedical applications. Antibacterial activity and mechanism of zno nanoparticles. Concerning the biosafety of nanotechnology, nanotoxicity is going to be the second most priority of. Although nanoparticles nps have made incredible progress in the field of nanotechnology and biomedical research and their applications are demanded throughout industrial world particularly over the past decades, little is known about the fate of nanoparticles in ecosystem. Good antimicrobial effects have been obtained from metal oxide nanoparticles raghunath and perumal, 2017, such as mgo, cu2o, cuo. In order to investigate new effective and inexpensive therapeutic approaches, we analyzed nanoparticles synthesized by a green approach using corresponding salt silver or zinc nitrate. Antibacterial activity of modified zinc oxide nanoparticles. Nanoparticles zinc oxide antibacterial antifungal reactive oxygen species. Cuo nanoparticles were also experimented for antibiofilm and time kill assay.
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